Category Archives: Enterovirus Lab Diagnosis

Comparing Molecular Methods for Early Detection and Serotyping of Enteroviruses in Throat Swabs of Pediatric Patients

Article published by POLS ONE on October 25,2012 ABSTRACT Background: Enteroviruses include over 100 serotypes and usually cause self-limited infections with non-specific symptoms in children, with the exceptions of polioviruses and enterovirus 71 which frequently cause neurologic complications. Therefore, early detection and serotyping of enteroviruses are critical in clinical management and disease surveillance. Traditional methods for detection and serotyping of… Read more »

Cross-reactive Neutralizing Antibody Responses to Enterovirus 71 Infections in Young Children: Implications for Vaccine Development

Article published by POLS ONE on February 14, 2013 Background: Recently, enterovirus 71 (EV71) has caused life-threatening outbreaks involving neurological and cardiopulmonary complications in Asian children with unknown mechanism. EV71 has one single serotype but can be phylogenetically classified into 3 main genogroups (A, B and C) and 11 genotypes (A, B1,B5 and C1,C5). In Taiwan, nationwide EV71 epidemics with… Read more »

CODEHOP-based RT-PCR (VP1- CODEHOP) for enterovirus infections detection and serotyping

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Article Published by POLS ONE on December 1, 2016 ABSTRACT Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1- CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveil- lance comparing the… Read more »